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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: N-Glycosylation Regulates ADAM8 Processing and Activation
doi: 10.1074/jbc.m114.594242
Figure Lengend Snippet: FIGURE 6. N91Q and N612Q ADAM8 mutants are unable to reach the cell surface. A, SUM-149 cells were transiently transfected with vectors express- ing WT ADAM8 or N67Q, N91Q, N436Q, or N612Q N-glycosylation mutants. Cells were fixed, permeabilized, and subjected to immunofluorescent stain- ing for ADAM8 expression using an anti-ADAM8 ectodomain antibody (AF1031) (red), and for the cell surface marker E-cadherin (green), and with DAPI (blue) to label the nuclei. Images were taken using a Nikon 80i Upright Research Fluorescence Scope (40 objective) with an Andoe Clara-E Camera andprocessedwithNIS-ElementsBasicsoftware.Scalebar,10m.B,HEK-293 cells were transiently transfected with vectors expressing WT ADAM8 or N-glycosylation mutants N91Q and N612Q. The cell surface proteins were labeled with biotin and pulled down by streptavidin (SA)-conjugated beads. Samples (10%) of extracts (Input) and pulled down biotinylated ADAM8 were detected by Western blotting using a Myc antibody.
Article Snippet: Images were taken using a
Techniques: Transfection, Glycoproteomics, Staining, Expressing, Marker, Fluorescence, Labeling, Western Blot